Extended use of biogas for city buses will lower emissions, improve inner city air quality and strengthen the role of public transport in an efficient strategy to limit the impact from traffic on climate change. This three year project will stimulate cities and regions around the Baltic Sea to use biogas driven buses. The project will generate strategies and policies to introduce biogas as well as analyse necessary measures in biogas production, distribution and bus operations. Activities will be executed to facilitate further expansion. Partners in the Baltic Sea Region will form a show room to demonstrate a sustainable transport system with the potential to reach the EU’s climate goals. The group of partners involved represents most of the countries within the Baltic Sea Region (BSR). The partnership offers an ideal platform for dissemination of knowledge, experience and technology. A broad set of associated organisations in each country will reinforce the processes of information. Through the pan-Baltic network the partners will obtain a better position to negotiate with infrastructure and bus suppliers and at the same time raise the visibility of biogas buses. Dependency on fossil fuels has tempered the interest for investment in new energy technologies, which makes public intervention to support energy innovation both necessary and justified. To make 2nd generation biofuels competitive to fossil fuels is a challenge – and biogas is the only commercially available 2nd generation biofuel. The Baltic Biogas Bus project aims to present cost effective solutions on biogas production as well as distribution and use in buses. The monitoring of economic and environmental impacts will demonstrate a renewable fuel for transport with excellent environmental performance. Efficient use of biogas buses depends on a process with several steps: production from biodegradable materials (waste, sewage sludge and landfill gas) including purification to get a gaseous fuel (biomethane) for vehicles and distribution, either by trailer or in pipelines. Since the characteristics of biogas are similar to natural gas it is possible to inject cleaned biogas into the natural gas grid, so creating a cost effective solution. Handling of biogas at the bus depots includes creating an optimal refuelling system: a fast filling system that fuels the bus as quick as diesel would be fuelled or a slow filling system fuelling the bus over night. The knowledge and experience from the project will form a bridge into the next generation of renewable fuels involving hydrogen. Consequently the project will analyse positive synergies in mixing biogas with hydrogen to get the most out of both renewable fuels.