Among the promising sectors identified in the cross-border region, health, agro-resources, and biotechnologies are those that have the most potential for development. However, the region is lagging behind in terms of research and development in comparison to national levels and European objectives. In order to encourage the introduction of developments in the context of these emerging themes and faced with the needs for filling or replacement raised by regenerative medicine, the TEXTOS project aims to offer surgeons in orthopaedics and odontology next generation substitutes that will allow wound healing and bone regeneration. The main objective of the project is, therefore, to develop an innovative 3D matrix for tissue engineering with the goal of reconstructing, regenerating, or replacing the tissue of defective organs. The prototype will be realised in two stages, firstly by developing a “core” matrix reflecting the proof of concept, and then the hybrid matrix of the target prototype. The TEXTOS project makes it possible to group and federate research and innovation stakeholders in the cross-border region around complementary themes. It represents an opportunity for the partners to share their practices and to increase their visibility at the national and international level in the field of tissue engineering.